Examine the nature of personal selling and the role of the sales force Essay

Examine the nature of personal selling and the role of the sales force - Essay Example Most sales happen as a result of advertising. Advertising could be placed in mediums such as television, newspapers, billboards, internet web-pages, etc. These forms of sales employ one-way communication, whereby messages are communicated from manufacturer to end-consumer with no immediate feedback. Personal selling, on the other hand, differs from conventional advertising, in that salespersons of a company build and maintain a long-term relationship with their customers. They do this by directly interacting with customers via phone or web conferences or in person. Many companies include personal selling as part of their overall sales strategy, especially if they foresee complex selling situations. In this type of selling â€Å"salespeople can probe customers to learn more about their problems, and then adjust the marketing offer and presentation to fit special needs of each customer.† (Kotler & Armstrong, p.453) Even within personal selling, a wide variety of strategies are e mployed by managers. For example, while companies that sell only online or through catalogs do not need any salespersons, the ones such as IBM or DuPont do need a sales force. In companies such as Procter & Gamble and Nike, the sales force â€Å"plays an important behind-the-scenes role.

On Finding Nightingales in Human Voices Essay Example for Free

On Finding Nightingales in Human Voices Essay Anne Finch’s â€Å"To the Nightingale† is an ode to a Muse, which is represented by the nightingale. The poem is written in a series of rhyming couplets that provides it a singsong rhythm throughout, which is appropriate for its subject. When emphasizing the merits of being a nightingale, the speaker articulates the difficulties of being a human poet, subject to judgment by his critics. In â€Å"To the Nightingale†, though the narrator recognizes the significance of the nightingale and even bestows upon it affirmative adjectives such as â€Å"sweet† and lofty titles like â€Å"harbinger of spring† (line 1), he knows the limitations of the bird as a poet. The first four lines indicate the admiration of the speaker for the nightingale. â€Å"This moment I attend to praise† (line 3) refers to the moment in which the nightingale will sing to announce the coming of spring. The speaker wishes to be as free with expressing himself, like the nightingale. As the line â€Å"Free as thine shall be my song† (line 5) indicates, he believes that as a human being, his singing is more restricted by his audience. On the other hand, the nightingale sings according to its nature, whether or not it pleases anyone. There is no fear of being ridiculed, or anxieties about not being praised. Moreover, the beauty of the nightingale’s singing is in its freedom: â€Å"Poets, wild as thee, were born/ Pleasing best when unconfined/ When to please is least designed/ Soothing but their cares to rest† (lines 7-10). These four lines may also suggest that the speaker is hoping to experience the same â€Å"unconfined† performance. Indeed, if the nightingale is his muse, he is inspired by its sheer autonomy and being true to itself. Some artists need their personal pain in order to produce depth of feeling. An artist who is experiencing problems while composing sad ballads will create genuine emotion which will be felt by their audience: â€Å"Cares do still their thoughts molest/ And still the unhappy poets breast, /Like thine, when best he sings, is placed against a thorn† (lines 11-13). The three lines, however, may also signify the other way around – that when at his best, a poet may experience loneliness brought by success. The next few lines incorporate gold as a metaphor for the beauty and the effect of the nightingale’s song, after praising the sweetness of it. â€Å"Canst thou syllables refine/Melt a sense that shall retain/Still some spirit of the brain† (lines 18-20). The words â€Å"refine† and â€Å"melt† elucidate the worth of the nightingale’s song. It can create something equivalent to gold, which consequently leaves a mark in the listener’s mind. The poem starts to change its tone by line 21. The speaker seems to expect more out of the nightingale, by asking it to change its note. He further commands â€Å"let division shake thy throat† (line 22), longing for the joyous varying and fluttering of the golden voice. At this point, the human poet, though still admiring the singing bird, becomes aware of discontent within himself. The lovely song may not have changed its tune for some other listener, but for the narrator it has in some way for he says â€Å"cease then, prithee, cease thy tune† (line 26). He even calls his muse â€Å"trifler†, or someone who takes nothing seriously by being a constant dreamer. â€Å"Wilt thou sing till June† (line 27), he asks. He previously tags the nightingale as a â€Å"harbinger of spring†. He then wonders if it will continue its singing even when summer is near. It is as if the nightingale has been given an obligation to announce spring, and when that obligation has been fulfilled there is no need to keep on going. The speaker has started to question inspiration and leans toward practicality. The speaker believes that there is too big a difference between a nightingale and a human poet; he has stopped dreaming about attempting to recreate the singing of a nightingale through human voice. â€Å"Thus we poets that have speech/ Unlike what the forests teach† (lines 30-31). To ease this discouragement, he lifts the human advantage of being able to speak. â€Å"If a fluent vein be shown/Thats transcendent to our own/ Criticize, reform, or preach/ Or censure what we cannot reach (lines 32-35). Nevertheless, he discerns what he is trying to do; he can identify the human trait of disparaging a talent or a quality that he cannot achieve for himself. â€Å"To the Nightingale† explores the dilemma of exploring a dream and aiming to reach its zenith without any thought of its limitations and consequences, and of choosing practicality and realistic aspirations. The poem achieves a light, song-like rhythm which prevents it from being completely dreary even at its despondent but sensible end.

Politics of the European Union: Literature Review

Politics of the European Union: Literature Review Nugent, N. (2003) The Government and Politics of the European Union, Palgrave. Nugent offers an analysis of the European Union in a historical context. He argues that many of the problems that sceptics tend to rely upon as criticism for EU membership were, in themselves, already present before the integration of the EU actually occurred. Many critics assume that the EU has significantly eroded and displaced the sovereignty of states. Nugent, however, posits that this occurred much earlier, and that integration into the EU cannot solely constitute the basis for erosion of sovereignty in nation-states. He suggests, in a historical analysis, that â€Å"the member states of the EU were seeing their sovereignties being steadily eroded long before the EC / EU was established† (1), and the rest of the book is informed by this view. He argues that the EU plays only a minor role in eroding state sovereignty, as broader economic factors such as movements in financial markets, multinational corporations and the general side-effects of dominance by the U.S. tend tow ard this model. In fact, Nugent suggests that the EU may in fact help to preserve autonomy in some ways because it provides a voice, albeit one marred by bureaucracy and corruption, that can compete economically with America and the emergent China. Nugent looks at the question of the EU in a historical way. He provides a historical analysis of state relations prior to the instigation of the EU. In this analysis, he insinuates that, while prior to the war states were notably more different in economic, cultural and in political ways, the period after the war signalled a significant shift in the ways the European states tended to interact. The integration of the EU was therefore defined by these factors, and present criticisms about the overwhelming bureaucracies that operate in Brussels merely represent something that is necessary. Bache, I George S (2006) The Politics of the European Union, 2nd Ed., Oxford University Press The Politics of the European Union provides a detailed and comprehensive overview of the operative and dynamic processes that determine how the EU functions from day to day. While some historical analysis is supplied, the focus is also focussed upon certain key issues in government. The book is split into five discrete sections. The first section looks at the theories of European integration, and essentially provides the background as to why European integration should (or shouldn’t) happen, dependent upon a number of different views. It also looks at how the European Union should be organised based upon these theories, and looks at ways in which the European Union should go in the future if it is to be adherent to these particular theories. Part two looks at the history of the European Union, and upon how certain developments in European politics led to the integration of the EU. Part three provides an atomised look at the various member states of the EU, with a particular fo cus upon Germany, France and Britain. Although other member states are mentioned in a brief chapter, the absence of analyses of other significant countries in the EU, and more detailed analysis of Eastern European, Spanish and Scandanavian member states gives the book a certain biases toward the previous three countries. Part four provides an interesting analysis of how the various institutions of the European Union operate, with a focus upon the nuts and bolts of the day to day functioning of policy change and implementation, rather than more detailed historical analysis. Finally, Part 5 looks at how the EU has implemented certain policies and why, ranging from its policies on agriculture, on the single market, on the monetary union and on external relations. Overall, the book provides a good textbook overview of the basic functioning, purpose, and theory behind the EU. Bomberg, E Stubb A. (2003), The European Union: How Does it Work?, Oxford University Press Again, The European Union: How Does it Work? provides an interesting and detailed analysis of the various ways in which the European Union has come to be what it is, and also focuses upon the institutional, theoretical and historical factors that have determined how and why it operates. The book is organised in a similar way to the previous book, insofar as it focuses first upon the historical and theoretical basis for European Integration, and then looks in more detail at the various policies that have been implemented, and the member states that act as players in the EU. Of particular importance is the analysis of how member states operate within the complex framework of the EU. Bomberg and Stubb concentrate upon the complexities of the EU, and try to rationalise the often overwhelmingly complicated issues at stake, using simple logical statements. They argue that, far from being an institution racked with bureaucracy which serves neither the interests of the EU nor the interests o f individual states, that the mechanisms and institutions in place create a series of checks and balances that allow the opinion of every participant state and political parties that operate within these states to function more appropriately. They argue that â€Å"What emerge as national interests from domestic systems of preference formation remain central to how the EU works†, and also suggest that what is implemented officially is also affected by considerable and sophisticated â€Å"horizontal networking† behind the scenes. This tendency to look at the actual, rather than the theoretical or institutional realities of the EU is a strength of this book, however, this intrinsically makes the project of the book more ambiguous and difficult to pinpoint. Rather than providing a coherent overview of the surfaces of the European Union, the book delves into the complexities and the awkward issues that inform and orient decisions surrounding policy, power and practice. Describe how Politics comes into the Process of European integration The process of European integration is a very complex one, and if a nation state chooses to integrate itself into the complex political arena of the European Union, one has to consider the effects that this will have upon the given state internally, and externally. In essence, the integration of European states means that a given state will take its interests from the domestic front and into the European Union. As Bomberg and Stubb (2003, p. 70) comment, â€Å"once a state joins the Union, politics may begin at home but no longer end there. National politics, polities, and policies become ‘Europeanized’.† As such, the externalisation of internal quandaries that, previously were a matter for the sovereign state, now have to be considered as an integral, institutional and political whole. While Nugent argues in The Government and Politics of the European Union that sovereignty was being eroded anyway before the processes of European integration took place, the polit ical processes that operated within nation states to deal with problems concerned with globalisation were not. A political climate emerges in the process of European integration as a result of conflicting or combined interests that interweave. Such issues as the integration into the single market, the single European currency, and agricultural policy levelled to prevent the overt exploitation of free markets and the production of substandard goods. The question of governance is also a complex political one regarding the EU, and the question of who governs shines light upon how politics tends to function and become a part of the process of European integration. The policy process of the EU is extraordinary in global political affairs, because it is not governed by a central body, moreover, it is governed by a series of nation states Stubb and Bomberg (2003, p. 148) comment that â€Å"No state or other international organization makes policies through such a complex, transnational process in which politicians, officials, and interested groups from across a continent interact to shape – sometimes to prevent – shared policy outcomes.† As such, politics becomes intertwined into European integration because of the melange of interests that operate under the umbrella of state, governmental, or political interest. Because no state, political or official group is in overall control of the policy making process, poli tics is essentially a part of European integration because it is via the institutions and the backroom political wrangling that the European Union makes its overall policy and political decisions. The political process in Europe enters the system through a variety of means. While supranational organisations tend to confirm political issues, it is often left to the member states and elected representatives of these states to conduct policy based upon how they would like political developments to proceed. The EU is an example of â€Å"networked governance†, and the ways in which the member states, individuals, pressure groups and other officials interact in the EU act to determine overall political policy. While a coherent political policy based upon the interests of these states tends to be cumbersome and bureaucratic, this is how politics tends to become instigated into the process of European integration. By becoming a member of the European Union, states have to recognise that their own sovereignty has been reduced by a political process that operates within a more European context. What are the challenges facing European integration today? The EU faces a number of significant challenges as it changes to adapt to new economic, political and supranational factors that determine and legitimate its efficacy as a political institution. Firstly, the expansion of the EU poses significant challenges for both existent member states and those that are new to the European Union. For instance, the integration of Eastern European member states such as Poland and the Czech Republic have proven to be controversial issues, because both these countries have a significantly different economy than those that are currently established. The challenges that face the EU is to consider the political climate of these emergent countries while making sure that the interests of those states currently in the European Union are considered. The policy processes and changes that take place in the European context must juggle these interests, while remaining firm to previous trade policies. As such, in the words of Bomberg and Stubb (p. 71), expansion and continued expansion into Eastern Europe and possibly Turkey and Cyprus, facilitates the importance of tolerance within the European Unions institutional framework: â€Å"managing difference is thus a key challenge to the Union.† Secondly, the economic challenges facing the European Union on a global basis will undoubtedly prove to be extremely important, especially following the successful implementation of the Euro into economic affairs. The EU is a significant global player on the economic field, and trade with the emergent countries of China and India as well as with established superpowers such as America and Japan have to be considered very carefully if successful relations are to be established. The presence of global aid programmes and other benevolent factors such as an easily mobilised team of peacekeepers is also an issue that is linked indirectly to processes of globalisation. The recent crisis in the Balkans was marred by the inability for the EU to make a coherent decision on troop assignment to the region. As such, issues of defence and aid may prove to be one of the central challenges facing the EU in the future. The relationship between the EU and the states outside of the EU may prove essential to determining a process whereby aid or trade can be granted to developing countries in a system of integrated change. The EU’s response to global crises has been far from efficient in recent years, and changes in the dynamic of the EU, which includes its expansion into regions of Eastern and Central Europe have further exacerbated tensions on this issue. While the EU have always been relatively generous in the giving of aid to other countries, the general trend posited in the WTO report is that the giving of aid is simply not enough to resolve problems on a global scale. Instead, the EU have to implement foreign trade more effectively into its policy, and, because of varying interests from its different participants, this may prove to be a stumbling block for more successful European integration. Lax spending programmes and bureaucracy concerning the giving of foreign aid may also hamper deve lopments in the global context: â€Å"the Commission had far to go before it escaped charges that it was the ‘worst development agency in the world’ (Bomberg Stubb 2003, p. 204). Does spill-over imply that there are no limits to the number of policies that can be dealt with at the European level? The concept of â€Å"spill-over† is defined as a process whereby the integration in one sphere of policy begets a residual impact in other spheres of policy, and creates a more generalised integrated series of policies in all areas of the European Union. For instance, the integration of agricultural policy in Europe tends to affect the internal policies of that sovereign state in ways that harmonise it with other nation states. Naturally, this facilitates the integration process because it allows for discrepancies and disagreements between various regions, officials and member states to be ironed out more generally. As such, it can be argued that this concept of ‘spill-over’ allows for significantly greater integration to occur, and the gradual homogenization of European member states may provide a forum by which all member states operate on a very similar basis. Therefore, by this method, all policy decisions can be discussed in the European Union. However, this system of spill-over is not without its flaws or its criticisms. For instance, political processes in some powerful member states that feel directly affected or marginalised by processes in the European government may not succumb to the integrative factors of spill-over, and regional, geographical and political factors still require consideration. While domestic policy is further eroded by the concept of spill-over, some tenets of policy that impede upon concepts of sovereignty, or perhaps indirectly attack or influence one particular region or nation-state operating within the framework of the European Union may disallow an invasive series of policy making decisions to be integrated into the European agenda. While a great many issues have been affected by the concept of spill-over, and the general process of unification that occurs as a result of spill-over into other policies on a European level tend toward a process of unification, some policies still remain too sens itive or regionalised to implement into European political processes. Bibliography Bache, I. George, S. (2006), The Politics of the European Union, 2nd ed., Oxford University Press. Bomberg E. Stubb A., ed. (2005), The European Union: How does it work?, Oxford University Press. Nugent, N. (2003), The Government and Politics of the European Union, Pelgrave.

Euro Disney the Failure Essay -- International Marketing Europe Theme

Walt Disney is the world leader in family entertainment and one of the most valuable brands in the world. Recently, Walt Disney has decided to open up a Disney Theme Park in Hong Kong China, which will be the first theme park in China. Since the opening of Euro Disney, the Walt Disney Company has learned to take into consideration important determinants so that their investment will not be a huge financial loss. Unfortunately, Since Disney did not research as much as they should of when deciding to open Euro Disney, this lead to one of the biggest failures for the Disney Company and is today used as an example of international marketing failures. Disney had high expectations about opening up a Disney Theme Park in France. They projected 500,000 visitors to be in the park on opening day and the result was only 20,000 visitors. According to the textbook, international corporations should research and analyze their profitability level and the market size. Many international companies need to expense their research and development because it will only lead to big financial losses. Walt Disney Company should of used a transnational approach to achieve a consistent and controlled marketing strategy. Transnational approach has three types of variables such as demographic, psychographic and behavioral. A big problem that Disney overlooked was the lack of marketing to the French people, there were more visitors from outside of France then there was in France. After realizing their turnover they starting promoting special package prices for French residents. Several fundamental points that affected the failure of Euro Disney's first 2 years were location, staffing, dining, prices and the opening of to many hotel rooms at ... ... for American companies to realize that "their way" isn't always going to work, especially in a different country with such a different culture. Their Hong Kong project seems like they are taking precaution to prevent any huge financial losses. In spite of their mistakes, Disney is still one of the most important brands in the world and is widely recognized as a successful corporation. Bibliography "Euro Disney: An American in Paris" http://www.business.bham.ac.uk/business/images/cme_resources/Users/Szmigin/euro%20isney.doc Businessweek.com. " Building a Mouse House in Hong Kong". April 11, 2002 http://www.businessweek.com/bwdaily/dnflash/apr2002/nf20020411_4498.htm Doole, Isobel and Lowe, Robin. "International Marketing Strategy". Third Edition, Copyright, 2001. Recklies, Dagmar. " Managing Resources-Accounting Assignment". Themanager.org

United States And Stroke Awareness Health And Social Care Essay

In the United States stroke has been reported as the 3rd prima cause of decease. There are about 795,000 instances of shots that are reported each twelvemonth in the United States. Over 143,579 of these people die each twelvemonth from holding a shot. Strokes can be really serious and consequence in decease if non treated instantly. There are several types of shots that one may hold and different hurts that may happen depending on what type of shot person has ( Center for Disease Control and Prevention, 2007 ) . There are besides different hazard factors associated with a shot. Some of these hazard factors can be controlled while some hazard factors are familial ( American Stroke Association, 2009 ) . A shot can happen in different ways. One manner is by holding parts of the encephalon blocked from the blood supply. When a blood vas explosions within the encephalon it can besides do a shot. Having blood supply blocked or a blood vas explosion causes harm to the encephalon. Stroke is the figure one cause of disablement that last for a long clip and can happen at any age. There are three different types of shots. Ischemic shot is one type, it occurs when an arteria is blocked that supplies blood and O to the encephalon. The most common cause of an arteria obstruction is blood coagulums. Hemorrhagic shot is another type of shot, which occurs when an arteria explosions in the encephalon. Hemorrhagic stroke can happen in several ways, such as ; an aneurism, which is a weak or thin country on an arteria wall that can spread out like a balloon. The thin walls of the arteria can tear or interrupt. Hemorrhagic stroke can besides happen when arterial walls lose their snap and go thin. Once an arterial wall becomes thin it can check and shed blood. Another hazard of a haemorrhagic shot is high blood force per unit area. Transeunt ischaemic onslaughts is another type of shot, it is sometimes called a mini-stroke. These mini-strokes start out like a shot, but clears up within 24 hours go forthing no symptoms. Having a transeunt ischaemic onslaught could be a warning that person could be at a higher hazard for a more serious shot. The sudden oncoming of transeunt ischaemic onslaught should signal an exigency. Patients who experience a transeunt ischaemic onslaught should have intervention instantly ( Center for Disease Control and Prevention, 2007 ) . Strokes affect people in different ways. Damage from a shot depends on the type of shot that person has. When a individual has a shot the encephalon may go injured doing many jobs, some countries of the encephalon green goods emotions, if these countries are injured a individual may shout easy or have sudden temper swings for no ground. Strokes can besides do a loss of consciousness. Peoples who have a shot may lose feeling in an arm or leg. If person has a shot on the right side of their encephalon, so their left side of their organic structure is affected. Stroke subsisters may bury about their left side which is their weaker side. A shot can besides impact seeing, touching, believing and traveling. A shot subsister ‘s perceptual experience of objects may be changed. Strokes can impact hearing and address every bit good. Stroke victims do non normally lose their hearing but have jobs with address and understanding address. Address jobs are associated when a individual has a s hot which affects the left side of the encephalon. Stroke victims besides may see jobs with masticating and get downing nutrient. This occurs when musculuss on one side of the oral cavity are weak. Another job with shots doing encephalon hurts are long-run and short-run memories. Stroke subsisters may non cognize how to get down a undertaking, or be confused why they walked into a room. After a shot, some of these jobs can be improved over clip ( American Heart Association, 2009 ) . There are several marks of a shot that people should be cognizant of. One mark is a sudden numbness of the weaponries, legs, or face. Another mark is sudden confusion. Having problem seeing out of either oculus or merely one oculus is another mark. Having problem with coordination, giddiness, or walking is another mark. Another mark is a sudden concern for no evident ground. All marks of shot can look all of a sudden, and sometimes there are more than one symptom go oning at the same clip. If person experiences any of these symptoms so immediate attending is required ( Center for Disease Control and Prevention, 2007 ) . There are different hazard factors associated with holding a shot. Some hazard factors are familial while other factors are from the manner a individual lives. Heredity hazard factors can non be changed, such as ; age. Opportunities for holding a shot are higher as person gets older ( Center for Disease Control and Prevention, 2007 ) . Two tierces of shots occur in people who are over 65. Some shots besides occur in people who are under 65. Other hazard factors that can non be changed are gender and racial group. Males have a higher hazard factor than females do and African Americans have a higher hazard factor than most other racial groups. A household history of shot and diabetes are besides risk factors that raise the hazard of holding a shot and can non be changed. High blood force per unit area is a hazard factor for shot that can be changed. Another hazard factor for shot is cigarette smoke, which can be changed. Diabetes is a hazard factor for a shot that can be controlled. Pe oples who have diabetes have other jobs, such as ; high blood force per unit area, high blood cholesterin, and are overweight. Having all these conditions increases the hazard even more. Artery disease increases the hazard of a shot. Atrial fibrillation, which changes the Black Marias beat, besides raises the hazard for shot. Heart failure, high blood cholesterin, and coronary bosom disease have a higher hazard of holding a shot. Poor diets can hold a function in a shot. Peoples who eat nutrients high in fat and cholesterin can raise their cholesterin degrees. Peoples who eat nutrients high in salt can increase their blood force per unit area. Other hazards of holding a shot that can be controlled are physical inaction and fleshiness. The lone ways to command some of the hazard factors are by eating a healthy diet. Eating a healthy diet can assist cut down three of the hazard factors associated with a shot, such as ; fleshiness, high blood cholesterin, and high blood force per unit area. Exerting mundane for at least 30 proceedingss can besides cut down the hazard of a shot. Keeping an oculus on blood force per unit area and non smoke will besides better person ‘s opportunities of non holding a shot ( American Stroke Association, 2009 ) . In the past interventions of a shot was merely basic life support at the clip of the shot and rehabilitation subsequently ( St. Luke ‘s Episcopal Hospital, 2008 ) . Timess have changed and people have realized that acquiring intervention for a shot instantly can cut down their encephalon harm and better their opportunities of lasting a shot. Treatment of a shot varies depending on what caused the shot. Before anyone can get down intervention a physician will execute several trial, such as ; a resonance imagination ( MRI ) or a computed imaging ( CT ) scan to name which type of shot occurred. Trials may besides be done to find where the hemorrhage is or to see where the coagulum is. Having these trials done will besides demo the Doctor ‘s how much encephalon harm has occurred. If an ischaemic shot is discovered within the first three hours when the symptoms started so a clot-dissolving medical specialty can be given, which can increase the opportunities of endurance and re covery. A individual whom had an ischaemic shot may besides be given an acetylsalicylic acid. Other medicine may be given to command febrility, ictuss, and blood sugar degrees. For a haemorrhagic shot medical specialties may be given to command febrility, ictuss, encephalon puffiness, blood sugar degrees, and blood force per unit area. Person who has a haemorrhagic shot will besides be watched closely for marks of force per unit area on the encephalon. If haemorrhagic shot is due to an aneurism so surgery may be done to mend it. After the first initial interventions are done for a shot so the focal point turns on rehabilitation and forestalling another shot. If a blood coagulum caused the shot so the patient may hold to take decoagulants to forestall another shot from go oning. Medicines may be given to stroke victims to take down high cholesterin or to command blood force per unit area. After a shot victim is stabilized so they may get down rehabilitation, which allows person who h ad a shot to recover some normal operation in their life. To cut down the hazard of holding another shot alterations in the life style of a shot victim will hold to be changed ( WebMD, 2009 ) . There are some future intervention options that are being researched to assist victims of a shot. One technique that is being researched involves the transplantation of research lab grown nervus cells into the encephalon of a shot victim. Research workers are trusting that by transfering these nervus cells into the encephalon of a shot victim that it will better motor and address accomplishments. This technique is still being researched. Since a shot is hard to handle research workers are looking at forestalling a shot before it happens ( St. Luke ‘s Episcopal Hospital, 2008 ) . On the Louisiana ‘s Department of Health and Hospitals ( 2006 ) , website there is plentifulness of information on what a shot is, what causes a shot, the hazard factors for a shot, and how to assist cut down the opportunity of holding a shot. The information on the web site is delivered in a manner that lets person understand precisely what is being said without utilizing large words. The information is delivered in an easy to read and apprehensible manner. The information is available to those who visit the Louisiana Department of Health and Hospitals office or web site. The information that the Louisiana Department of Health and Hospitals offer is really helpful and comprehensive. Explaining how shots occur and how to forestall a shot is the most of import information that is available. There is besides a subdivision on bosom healthy formulas. These different formulas are of import when person needs to cognize precisely what is healthy to eat, and what can take down their ha zard of holding a shot. The Louisiana Department of Health and Hospitals needs to supply a subdivision on how shots are treated. There besides needs to be local support groups in every parish in Louisiana. The Mayor of Louisiana has set aside as the month of May being stroke month consciousness, but more demands to be done. Many people are non cognizant of the stroke month consciousness. Peoples are besides non cognizant that they can travel to their local Health Department and acquire information and aid with shot ( Louisiana Department of Health and Hospitals, 2006 ) . To suggest a new and effectual manner to acquire the word out about the improved plan one should utilize an mean individual on a flyer discoursing what a shot is, marks and symptoms of a shot, the hazard factors for a shot, and bar methods for a shot. This flyer should be mailed to occupants in each parish who are in-between aged and older. The flyers should besides hold information about their local Health Departments, and who they may reach if they have any inquiries or concerns about a shot. These flyers should be sent out allowing occupants know about the shot awareness month, and that they can come in and acquire a free blood force per unit area cheque, discourse eating healthy and how regular exercising helps lower their hazard of a shot. Strokes can happen at any age, but as person gets older the opportunities of holding a shot additions. To cut down the hazard of holding a shot one should avoid smoke and eat a healthy diet. If person has diabetes so proper medicine and intervention should be taken to assist take down the opportunity of holding a shot. Exerting on a regular basis on a day-to-day footing will besides assist lower the opportunity of holding a shot. If one has a shot so immediate medical attending is of import within the first three hours, which can take down encephalon harm and aid with recovery. Being cognizant of the hazard factors that one can command will assist cut down the hazard of holding a shot ( American Stroke Association, 2009 ) .

Free Essays on Institutions

There is no doubt that the set of practices associated with â€Å"democracy† were first seen in the West. Most of these democratic nations such as the United States and Britain were strong enough to spread influence to eastern countries such as Japan and even China. There are, however, questions that arise as to why these democratic practices have been set up and even copied throughout the world. The biggest questions dealing with this situation are, is a democracy so much better than other political and governmental setups in the world, and if so, why? To estimate the performance of any political or legal institutions in society, many factors must first be considered. In a successful political and legal institution, all of these factors are present. In a prosperous institution such as the United States there is a clearly defined middle class. While in Brazil there is a rich class and a poor class, with no clearly labeled group of middle class people. A lack of black markets is a factor that promotes political and legal success. Although the United States does have black markets, there are a low number of them, and they are not as numerous or harmful to businesses or people as the black markets in Brazil. In successful institutions, there is always an established, fair judicial system. Here in the United States we have the court system, and an ethical police force, but in countries such as Mexico, courts may be corrupted, and police often try to extort people and even businesses. Another factor that is found in these succes sful institutions is a representative government, and a public that has faith in its government and its systems. These factors are found in the United States, but in many other countries such as Cuba, these systems are replaced by a dictator and a public that only shows a small amount of morale and faith in their government. In the politically and legally successful United States, the economy is healthy ... Free Essays on Institutions Free Essays on Institutions There is no doubt that the set of practices associated with â€Å"democracy† were first seen in the West. Most of these democratic nations such as the United States and Britain were strong enough to spread influence to eastern countries such as Japan and even China. There are, however, questions that arise as to why these democratic practices have been set up and even copied throughout the world. The biggest questions dealing with this situation are, is a democracy so much better than other political and governmental setups in the world, and if so, why? To estimate the performance of any political or legal institutions in society, many factors must first be considered. In a successful political and legal institution, all of these factors are present. In a prosperous institution such as the United States there is a clearly defined middle class. While in Brazil there is a rich class and a poor class, with no clearly labeled group of middle class people. A lack of black markets is a factor that promotes political and legal success. Although the United States does have black markets, there are a low number of them, and they are not as numerous or harmful to businesses or people as the black markets in Brazil. In successful institutions, there is always an established, fair judicial system. Here in the United States we have the court system, and an ethical police force, but in countries such as Mexico, courts may be corrupted, and police often try to extort people and even businesses. Another factor that is found in these succes sful institutions is a representative government, and a public that has faith in its government and its systems. These factors are found in the United States, but in many other countries such as Cuba, these systems are replaced by a dictator and a public that only shows a small amount of morale and faith in their government. In the politically and legally successful United States, the economy is healthy ...

Free Essays on Occupational Risk

Occupational Risk Thesis Whatever the profession, or trade, one may be involved in, a person deserves the right to a safe workplace, and the right to know about the dangers they may encounter during the course their work. These dangers includes, but are not limited to, providing information regarding faulty tools, irregular working conditions, malfunctioning equipment, hazardous chemicals, or everyday precautionary methods that must be taken in order to guarantee safety. In the same sense, workers should have the right to refuse unsafe work, or work in unsafe conditions. Workers should also be granted the right to participate in decisions that affect workplace health and safety. Finally, training courses should be held in order to educate employees on the dangers of the workplace, and so they may receive instructions on how to handle hazardous situations, or materials, properly. Right to a Safe Workplace What is the right to a safe workplace? This is merely being provided working conditions that are free of recognizable hazards. Working in unsafe conditions threatens a person’s survival, and ability to provide for themselves and their family. Without the right to a safe workplace, â€Å"all other rights lose their significance.† Right to know, Right to Refuse Unsafe Conditions The right to know, and to refuse unsafe working conditions, is different from the right to a safe workplace. The right to know is actually a compilation of several rights that extend from duties of the employer. The duties are as follows: â€Å"1) the duty to reveal information already possessed, 2) the duty to communicate information about hazards through labeling, written communication, and training programs, 3) the duty to seek out existing information from the scientific literature and other sources, and 4)the duty to produce new information relevant to employee health.† In order for an employee to refuse work they believe to be haza... Free Essays on Occupational Risk Free Essays on Occupational Risk Occupational Risk Thesis Whatever the profession, or trade, one may be involved in, a person deserves the right to a safe workplace, and the right to know about the dangers they may encounter during the course their work. These dangers includes, but are not limited to, providing information regarding faulty tools, irregular working conditions, malfunctioning equipment, hazardous chemicals, or everyday precautionary methods that must be taken in order to guarantee safety. In the same sense, workers should have the right to refuse unsafe work, or work in unsafe conditions. Workers should also be granted the right to participate in decisions that affect workplace health and safety. Finally, training courses should be held in order to educate employees on the dangers of the workplace, and so they may receive instructions on how to handle hazardous situations, or materials, properly. Right to a Safe Workplace What is the right to a safe workplace? This is merely being provided working conditions that are free of recognizable hazards. Working in unsafe conditions threatens a person’s survival, and ability to provide for themselves and their family. Without the right to a safe workplace, â€Å"all other rights lose their significance.† Right to know, Right to Refuse Unsafe Conditions The right to know, and to refuse unsafe working conditions, is different from the right to a safe workplace. The right to know is actually a compilation of several rights that extend from duties of the employer. The duties are as follows: â€Å"1) the duty to reveal information already possessed, 2) the duty to communicate information about hazards through labeling, written communication, and training programs, 3) the duty to seek out existing information from the scientific literature and other sources, and 4)the duty to produce new information relevant to employee health.† In order for an employee to refuse work they believe to be haza...

Frances Perkins, First Woman in a Presidential Cabinet

Frances Perkins, First Woman in a Presidential Cabinet Frances Perkins (April 10, 1880 - May 14, 1965) became  the first woman to serve in a presidents cabinet when she was appointed the Secretary of Labor by  Franklin D. Roosevelt. She played a prominent public role throughout Roosevelts 12-year presidency and was instrumental in shaping New Deal policies and major pieces of legislation such as the Social Security Act. Early Life and Education Fannie Coralie Perkins (she would later adopt the first name Frances) was born in Boston, Massachusetts, on April 10, 1880. Her family could trace its roots back to settlers in the 1620s. When she was a child, Perkins father moved the family to Worcester, Massachusetts, where he operated a store that sold stationery. Her parents had little formal education, but her father, in particular, read widely and had educated himself about history and the law. Perkins attended Worcester Classical High School, graduating in 1898. At some point in her teen years, she read How the Other Half Lives by Jacob Riis, the reformer and pioneering photojournalist. Perkins would later cite the book as an inspiration for her lifes work. She was accepted to Mount Holyoke College, though she was fearful of its rigorous standards. She had not considered herself to be very bright, but after working hard to pass a challenging chemistry class, she gained self-confidence. As a senior at Mount Holyoke,  Perkins took a course on American economic history. A field trip to local factories and mills was a requirement of the course. Witnessing firsthand the poor working conditions had a profound effect on Perkins. She realized that workers were being exploited by dangerous conditions, and came to see how injured workers could find themselves forced into a life of poverty. Before leaving college, Perkins helped found a chapter of the National Consumers League. The organization sought to improve working conditions by urging consumers not to purchase products manufactured in unsafe conditions.   Career Beginnings After graduation from Mount Holyoke in 1902, Perkins took teaching jobs in Massachusetts and lived with her family in Worcester. At one point, she rebelled against her familys wishes and traveled to New York City to visit an agency which dealt with helping the poor. She insisted on getting a job interview, but wasnt hired. The director of the organization thought she was naive and presumed that Perkins would be overwhelmed working among the urban poor. After two unhappy years in Massachusetts after college, Perkins applied and was hired for a teaching job at Ferry Academy, a girls boarding school  in Chicago. Once settled in the city, she began visiting Hull House, a settlement house founded and led by noted social reformer Jane Addams. Perkins changed her name from Fannie to Frances and devoted all the time she could to her work at Hull House. After three years in Illinois, Perkins took a job in Philadelphia for an organization that researched social conditions faced by young women and African Americans working in the citys factories. Then, in 1909, Perkins earned a scholarship to attend graduate school at Columbia University in New York City. In 1910, she completed her masters thesis: an investigation of undernourished children attending a school in Hells Kitchen. While completing her thesis, she began working for the New York office of the Consumers League and became active in campaigns to improve working conditions for the citys poor. Political Awakening On March 25, 1911, a Saturday afternoon, Perkins was attending a tea at a friends apartment on Washington Square in New Yorks Greenwich Village. The sounds of a terrible commotion reached the apartment, and Perkins raced a few blocks to the Asch Building on Washington Place. A fire had broken out at the Triangle Shirtwaist Factory, a clothing sweatshop that employed mostly young immigrant women. Doors kept locked to prevent workers from taking a break trapped the victims on the 11th floor, where ladders of the fire department couldnt reach them. Frances Perkins, in the crowd on a nearby sidewalk, witnessed the horrible spectacle of young women falling to their deaths to escape the flames. The conditions in the factory cost 145 lives. Most of the victims were young working class and immigrant women. The New York State Factory Investigation Commission was formed within months of the tragedy. Frances Perkins was hired as an investigator for the commission, and she was soon leading inspections of factories and reporting on safety and health conditions. The job was aligned with her career goal, and it brought her into a working relationship with Al Smith, a New York City assemblyman who served as the vice-chair of the commission. Smith would later become governor of New York and eventually the Democratic nominee for president in 1928. Political Focus In 1913, Perkins married Paul Caldwell Wilson, who worked in the office of the mayor of New York City. She kept her last name, partly because she was often giving speeches advocating better conditions for workers and she didnt want to risk that her husband would be embarrassed. She had a child that died in 1915, but a year later gave birth to a healthy baby  girl. Perkins assumed she would ease away from her work life and devote herself to being a wife and mother,  perhaps volunteering for various causes. Perkins plan to withdraw from public service changed for two reasons. First, her husband began to suffer bouts of mental illness, and she felt compelled to stay employed. Second, Al Smith, who had become a friend, was elected governor of New York in 1918. It seemed to Smith that women would soon get the vote, and it was a good time to hire a woman for a substantial role in the state government. Smith appointed Perkins to the industrial commission of the New York State Department of Labor.   While working for Smith, Perkins became friends with Eleanor Roosevelt, and her husband, Franklin D. Roosevelt. As Roosevelt was recuperating after contracting polio, Perkins helped him keep in touch with labor leaders and began to advise him on the issues. Appointed by Roosevelt After Roosevelt was elected governor of New York, he appointed Perkins to head the New York State Department of Labor. Perkins was actually the second woman to be in a New York governors cabinet (in Al Smiths administration, Florence Knapp had served briefly as secretary of state). The New York Times noted that Perkins was being promoted by Roosevelt as he believed she had made a very fine record in her post in the state government. During Roosevelts term as governor, Perkins became nationally known as an authority on laws and regulations governing labor and business. When an economic boom ended and the  Great Depression began in late 1929, less than a year into Roosevelts term as governor, Perkins faced a startling new reality.  She immediately began making plans for the future. She took actions to deal with the impact of the Depression in New York State, and she and Roosevelt essentially prepared for how they could take action on a national stage. After Roosevelt was elected president in 1932, he appointed Perkins to be the nations secretary of labor, and she became the first woman to serve in a presidents cabinet.   Role in The New Deal Roosevelt took office on March 4, 1933, stating Americans had nothing to fear but fear itself. The Roosevelt administration immediately went into action to battle the effects of the Great Depression. Perkins led the effort to institute unemployment insurance. She also pushed for higher wages for workers as a measure to stimulate the economy. One of her first major actions was to oversee the creation of the Civilian Conservation Corps, which became known as the CCC. The organization took young unemployed men and put them to work on conservation projects throughout the nation. Frances Perkins  greatest achievement is generally considered her work devising the plan that became the Social Security Act. There was great opposition in the country to the idea of social insurance, but the act successfully passed  through Congress and was signed into law by Roosevelt in 1935. Decades later, in 1962, Perkins gave a speech titled The Roots of Social Security in which she detailed the struggle: Once you get the ear of a politician, you get something real. The highbrows can talk forever and nothing happens. People smile benignly  on them and let it go. But once the politician gets an idea, he deals in getting things done. In addition to her work shaping  legislation, Perkins was at the center of labor disputes. In an era when the labor movement was approaching its peak of power, and strikes were often in the news, Perkins became extremely active in her role as labor secretary. Impeachment Threat In 1939,  conservative members of Congress,  including Martin Dies, the leader  of the  House Committee on Un-American Activities, launched a crusade against her. She  had prevented the speedy deportation of an Australian-born leader of the West Coast longshoremans union, Harry Bridges. He had been accused of being a communist. By extension, Perkins was accused of communist sympathies. Members of Congress moved to impeach Perkins in January 1939, and hearings were held to decide whether impeachment charges were warranted. Ultimately, Perkins career withstood the challenge, but it was a painful episode. (While the tactic of deporting labor leaders had been used before, evidence against Bridges fell apart during a trial and he remained in the United States.) Outbreak of World War II On December 7, 1941, Perkins was in New York City when she was told to return to Washington immediately. She attended a cabinet meeting that night at which Roosevelt told his administration about the severity of the attack on Pearl Harbor.   At the beginning of World War II, American industry was transitioning from producing consumer goods to the material of war. Perkins continued as secretary of labor, but her role was not as prominent as it had been previously. Some of her major goals, such as a national health insurance program, were abandoned. Roosevelt felt he could no longer spend political capital on domestic programs. Perkins, exhausted by her long tenure in the administration, and feeling that any  further goals were unattainable, planned to leave the administration by 1944. But Roosevelt asked her to stay after the election of 1944. When he won a fourth term, she continued on at the Labor Department. On April 12, 1945, a Sunday afternoon, Perkins was at home  in Washington when she  received an urgent call to go to the White House. Upon arrival, she was informed of President Roosevelts death. She became determined to leave government, but continued in a transition period and stayed in the Truman administration for a few months, until July 1945. Later Career and Legacy President Harry Truman later asked Perkins  to return to government. She took a post as one of three civil service commissioners overseeing the federal workforce. She continued in that job until the end of the Truman administration. Following her long career in government, Perkins remained active. She taught at Cornell University, and often spoke about government and labor topics. In 1946, she published a book, The Roosevelt I Knew, which was a generally positive memoir of working with the late president. However, she never published a full account of her own life. In the spring of 1965, at age  85, her health began to fail. She died on May 14, 1965 in New York City. Notable political figures, including President Lyndon Johnson, issued tributes to her and to her work that helped bring America back from the depths of the Great Depression. France Perkins Fast Facts Full Name:  Fannie Coralie PerkinsKnown As:  Frances PerkinsKnown For: First woman in a presidents cabinet; major figure in the  passage of Social Security; trusted and valued adviser to President Franklin D. Roosevelt.Born:  April 10,1880 in  Boston, Massachusetts.Died: May 14,1965 in New York, New YorkSpouses Name: Paul Caldwell WilsonChilds Name: Susana Perkins Wilson Sources Frances Perkins. Encyclopedia of World Biography, 2nd ed., vol. 12, Gale, 2004, pp. 221-222. Gale Virtual Reference Library.Perkins, Frances. Great Depression and the New Deal Reference Library, edited by Allison McNeill, et al., vol. 2: Biographies, UXL, 2003, pp. 156-167. Gale Virtual Reference Library.Perkins, Frances. American Decades, edited by Judith S. Baughman, et al., vol. 5: 1940-1949, Gale, 2001. Gale Virtual Reference Library.Downey, Kirstin. The Woman Behind the New Deal. Doubleday, 2009.

Uneployment Assignment Example | Topics and Well Written Essays - 2500 words

Uneployment - Assignment Example extreme levels to an extent of posing major security threats to the respective countries since unemployment is recognized as the mother of courtliness ills like dishonesty, corruption and falsehood. It makes humans develop the dark side of their character. This paper is going to explore the issue of unemployment into by first describing the link between the unemployment rate and the GDP growth rate in Greece and in Australia during the period 2003-2012; explaining causes of structural unemployment in Nigeria as well as evaluating policies adopted by the coalition government since 2010 to reduce unemployment in the UK. Using relevant statistics, describe the link between the unemployment rate and the GDP growth rate in Greece and in Australia during the period 2003-2012 ( You are not expected to compare the two countries Sources show that Australia’s unemployment rate began to climb at the same time carbon tax started to increase. The carbon tax began on July 2012 and since then the rate of unemployment that had been dormant for two years started increasing dramatically. Australia’s GDP is said to have been outperforming that of major developed countries in the year 2011 and 2012 since its economy was accelerating upwards an impressive rate not until the carbon tax was implemented. In 2012, the rate of unemployment in Australia was 5.2% Unemployment in Nigeria is among the most critical problems that the country has been facing for many years. The rate of unemployment in the country is at alarming levels despite the fact that Nigeria is endowed with diverse and infinite resources, both human and material. According to a study that was conducted in 2010, the secondary-school graduates consisted of the principal fraction of the unemployed accounting for nearly 35% to 50% with the average the rate of unemployment within the age group of 20 to 24 years being 40 % while that for persons between 15 to 19 years was found to be 31 %. There exists a number of

Career Profile - Computer Programmer Essay Example | Topics and Well Written Essays - 1000 words

Career Profile - Computer Programmer - Essay Example When I thought of who I would interview about computer programming, Matt came to mind. He is a senior system operation manager at Dixie Electric Company. He has been a computer programmer for 25 years since his graduation from the Massachusetts University of Technology. He stood five feet tall, charming and very jovial. I initially met him during graduation party of one of my friends, where he was the main speaker. He gave a keynote address that forever ringed in me. How could I forget him? On this particular day, I meet him in his office. ‘Hello, Shen! It’s been a while. How are you fairing on?† He said as he welcomed me into his spacious office. It looked quite expensive.Gold linings donned the walls, with hanging frames of awards of his exemplary performance. â€Å"He must be living well,† I said to myself. After exchanging pleasantries, we delved into the subject matter. He acknowledged that his interest in computers began way back during his days in hig h school. During his senior year in high school, he decided to pursue computer programming as a career. He notes that most employers look for programmers who have had at least four-year training in programming. He reaches out to two files of applicants that sought an opportunity at the firm. â€Å"These are potential candidates who may have their application considered, â€Å"he interjects as he hands me the files to have a quick look. Matt is a strict person and a perfectionist. He always means what he says. What was his motivation in computers?

Virtualization Across the Board Assignment Example | Topics and Well Written Essays - 1000 words

Virtualization Across the Board - Assignment Example ehensive performance metrics was done on an environment that had 38 physical servers, and x-servers that were providing a variety of services like running an oracle database. On analysis, it was realized that some servers were used constantly while others were idle for some large amounts of time. This research has therefore been able to highlight some hardware was inefficiently used, and thus proved some worthy points on the advantages of using hardware virtualisation as discussed below. On cost management, the study proved that cost savings could be increased by using some of the virtualization products offered by Oracle. In this case, the number of physical servers could be reduced from 38 to 10 (sagelogix, 2011). This reduction in physical servers would therefore ensure that management of the systems would be easier. Cost would be taken into consideration as the reduction of the physical servers would ensure that the data centre would reduce in size by 78%. This would additionally save on costs through reduced electricity bills, space bills and requirements for cooling. Managing of the system would be simplified by the above factors. Cost of licensing all the servers would also be greatly reduced (sagelogix, 2011). It is thus wise that hardware virtualization is a real business asset. The system architecture which then becomes homogenously interconnected would greatly improve the scalability of the system. This would be due to the fact that it would be simpler to load applications through the Oracle Virtual machine, which is highly available, at no additional costs in terms of hardware and software. A uniform architecture and a uniform operating system would easily improve the management of the system (sagelogix, 2011). Software virtualization is double edged. It has brought quite some changes in the way businesses run their trade and improved their services, and at the same time has had an impact on the way vendors licence their wares. According to Greenward

Allocation of Funds in the DOE Essay Example | Topics and Well Written Essays - 1750 words

Allocation of Funds in the DOE - Essay Example This document will show the reasons for the re-allocation of the monies toward viable sources of energy, and will also outline the importance of energy conservation as a tool in adjusting from hydrocarbon fuel sources to the alternative energy source formats. Current Distribution of Funds, in millions, for 2011 Projected Distribution of Funds, in millions, for 2012 $100M Nuclear Energy $100M Nuclear Energy $145M Fossil Fuels $45M Fossil Fuels, until projected phase-out $75M Alternative Energy Sources ($40M for Hydrogen and Fuel Cells) ($90M for Hydrogen and Fuel Cells) ($15M for Solar Energy) ($10M for Wind) ($25M for Wind) ($10M for Biomass) ($0 for Water Power) ($40M for Water Power) $30M Energy Conservation and Weatherization $50M Energy Conservation and Weatherization Our planet is in crisis. We are technologically more advanced than ever, but our advancement comes at a price. We need to face the reality that our energy consumption is far exceeding the fuel resources we are using to sustain our lifestyle. Wood, coal, petroleum - each resource we have employed since we discovered how to turn energy into power has become a part of the global problem which it is now too late to solve: how do we save our planet from ourselves? In reviewing the alternatives for energy sources and the technologies currently exploring those alternatives it is my recommendation, based upon the book The End of Oil by Paul Roberts, that the following areas merit the bulk of the available funds: Energy Conservation and Weatherization, Nuclear Energy, and of the alternative energy sources that have been explored, Hydrogen and Fuel Cells. The abundance of a given resource is a deciding factor in determining which technologies merit funding. The question is which resource will carry us the farthest, making it the most worthy of investment? Let us consider the merits of the various resources, starting with coal, which falls under the category of fossil fuels. Coal was literally the corner stone of the Industrial Revolution at the turn of the last century. (27, Roberts) However, in the twenty-first century, with a century of pollution having adulterated our environment, Roberts points out that we have no choice but to consider cleaner sources of energy. Coal did, however, replace wood as an energy source, and it was able to achieve a technological leap previously impossible with wood: â€Å"Newcomen’s engine took the process one step further, by converting the heat energy from combustion into the physical, or mechanical, energy of work.† (28) Still, as a renewable energy source, it is found lacking. Coal and other fossil fuels are finite resources, making them unworthy of further financial investment. The Department of Energy is hereby charged with managing the re-allocation of funds to renewable sources of energy. The Department of Energy must also utilize the re-allocated funds in the Energy Conservation category to maximum advantage, for the following reason: â€Å"Changing our energy sources has historically been a highly disruptive process.† (15, author) This is to say that whenever people have negotiated the transition from one energy source to another, the change is slow to take effect because the existing technology works best with the resources currently

British Petroleum Company Assignment Example | Topics and Well Written Essays - 1250 words

British Petroleum Company - Assignment Example This paper highlights that Shareholders and owners of BP have the major influence on other stakeholders and business. The cost cutting process may also be a direct result of their emphasis on reducing expenses so that profitability could be improved. Managers are the people who have been trusted with the responsibility of managing the day to day affairs of the firm. Managers of BP are persons responsible for ensuring that the manufacturing facilities at its exploration facility are in conducive form and do not pose any threat to the life of labors or others. Managers interact with the business on daily basis because managing the business is their day to day job. They also interact with other stakeholders through annual general meetings, board meetings as well as interacting with government agencies. The decisions of the managers directly affect the business and the managers as in this case. Managers decided to cost cut and reduce the expenses however; this has resulted into the significant risk issues for the firm. From this paper it is clear that employees are the individuals who work for the organization against certain wages. Employees for BP are the people who work at its exploration facilities and were hurt in different incidents due to security and risk lapses. Business partners of the firm are those firms or individuals who do business with the firm i.e. suppliers of raw material as well other material. Business partners of BP are American government, different other suppliers who have provided it resources to extract the oil from its Texas oilfield. They can influence the business and other stakeholders to the extent of their involvement in the overall affairs of the firms. In terms of safety and risk, business partners can only be limited to the installation and maintenance services provided by them.  

Strategic Environmental Assessment Essay Example | Topics and Well Written Essays - 750 words

Strategic Environmental Assessment - Essay Example Strategic environment assessment in general is defined as a, systematic and proactive process which helps in evaluating the policies and programs and comes out with the possible consequences on the environment. While assessing the impacts a considerate view is also taken of its economic and social considerations as well. It is worthwhile here to mention that strategic environment assessment (SEA) takes precedence over the EIA i.e. project environmental impact assessment (UNECE, 2003). A number of water tributaries merging into a main stream with the help of an area of land called watershed holds key to the availability of water for the that region. Watershed management calls for maintaining an eye on the quality and quantity of water resources and initiating proactive efforts for preserving these resources for longer durations. It essentially involves planning the efforts, collecting the data from different sources, assessing and evaluating the data, development of requisite strategies and then implementation of the strategies. Watershed management strategies differ from region to region depending upon the topography of the terrain and availability of the resources. If due attention is not accorded to the watershed region, situation may go out of hand in due course. For example, inflows into the Saguling reservoir in Indonesia have been decreasing over a period of time. Due to watershed degradation the dry season flow into the reservoir was found to be around 38 percent of the average annual flow between 1986 and 1991. This percentage went further down to 36 percent between 1992 and 1997 and to 34 percent in 1998 (ADB, 2006). Therefore SEA helps in preparing an early warning system for taking care of the environmental concerns. With an effective environmental policy and legislative framework in place, Thailand has been paying due attention towards its environmental requirements and focusing on environmental and energy concerns at the national and provincial levels. But, the environment management faces a stiff challenge in view of the rapid industrialization in the country. The country is mainly divided into 7 river basins with further sub-division into 25 drainage areas1. In order to manage the watershed region, the river basins and drainage areas have to be kept in good shape so that the charging system of earth is not blocked. Thailand has forestland stretching over 2000 kilometers from north to south with varied climatic conditions and a coastline. The clear and muddy waters too lap productive mudflats (Datamonitor, 2009). The Strategic Environmental Assessment (SEA) is therefore meant to serve as a promotional tool for expanding the adoption of policies towards preserving the watershed areas. Thailand shares three rivers with its neighbors Myanmar, Malaysia and the Lao People's Democratic Republic. The strategic environment assessment calls not only for assessing the status of watershed areas, but it also requires the experts to consider the management of all the relevant stakeholders. Some of the key stakeholders in this venture include landowners, local businesses, Farmers, industries, city officials, environmental activists etc. The diversity of these groups and their interests often creates a situation where it becomes very difficult to carry out the maintenance of the land, vegetation and water resources of the drainage basin. If the strategic policies are

Allocation of Funds in the DOE Essay Example | Topics and Well Written Essays - 1750 words

Allocation of Funds in the DOE - Essay Example This document will show the reasons for the re-allocation of the monies toward viable sources of energy, and will also outline the importance of energy conservation as a tool in adjusting from hydrocarbon fuel sources to the alternative energy source formats. Current Distribution of Funds, in millions, for 2011 Projected Distribution of Funds, in millions, for 2012 $100M Nuclear Energy $100M Nuclear Energy $145M Fossil Fuels $45M Fossil Fuels, until projected phase-out $75M Alternative Energy Sources ($40M for Hydrogen and Fuel Cells) ($90M for Hydrogen and Fuel Cells) ($15M for Solar Energy) ($10M for Wind) ($25M for Wind) ($10M for Biomass) ($0 for Water Power) ($40M for Water Power) $30M Energy Conservation and Weatherization $50M Energy Conservation and Weatherization Our planet is in crisis. We are technologically more advanced than ever, but our advancement comes at a price. We need to face the reality that our energy consumption is far exceeding the fuel resources we are using to sustain our lifestyle. Wood, coal, petroleum - each resource we have employed since we discovered how to turn energy into power has become a part of the global problem which it is now too late to solve: how do we save our planet from ourselves? In reviewing the alternatives for energy sources and the technologies currently exploring those alternatives it is my recommendation, based upon the book The End of Oil by Paul Roberts, that the following areas merit the bulk of the available funds: Energy Conservation and Weatherization, Nuclear Energy, and of the alternative energy sources that have been explored, Hydrogen and Fuel Cells. The abundance of a given resource is a deciding factor in determining which technologies merit funding. The question is which resource will carry us the farthest, making it the most worthy of investment? Let us consider the merits of the various resources, starting with coal, which falls under the category of fossil fuels. Coal was literally the corner stone of the Industrial Revolution at the turn of the last century. (27, Roberts) However, in the twenty-first century, with a century of pollution having adulterated our environment, Roberts points out that we have no choice but to consider cleaner sources of energy. Coal did, however, replace wood as an energy source, and it was able to achieve a technological leap previously impossible with wood: â€Å"Newcomen’s engine took the process one step further, by converting the heat energy from combustion into the physical, or mechanical, energy of work.† (28) Still, as a renewable energy source, it is found lacking. Coal and other fossil fuels are finite resources, making them unworthy of further financial investment. The Department of Energy is hereby charged with managing the re-allocation of funds to renewable sources of energy. The Department of Energy must also utilize the re-allocated funds in the Energy Conservation category to maximum advantage, for the following reason: â€Å"Changing our energy sources has historically been a highly disruptive process.† (15, author) This is to say that whenever people have negotiated the transition from one energy source to another, the change is slow to take effect because the existing technology works best with the resources currently

Strategic Environmental Assessment Essay Example | Topics and Well Written Essays - 750 words

Strategic Environmental Assessment - Essay Example Strategic environment assessment in general is defined as a, systematic and proactive process which helps in evaluating the policies and programs and comes out with the possible consequences on the environment. While assessing the impacts a considerate view is also taken of its economic and social considerations as well. It is worthwhile here to mention that strategic environment assessment (SEA) takes precedence over the EIA i.e. project environmental impact assessment (UNECE, 2003). A number of water tributaries merging into a main stream with the help of an area of land called watershed holds key to the availability of water for the that region. Watershed management calls for maintaining an eye on the quality and quantity of water resources and initiating proactive efforts for preserving these resources for longer durations. It essentially involves planning the efforts, collecting the data from different sources, assessing and evaluating the data, development of requisite strategies and then implementation of the strategies. Watershed management strategies differ from region to region depending upon the topography of the terrain and availability of the resources. If due attention is not accorded to the watershed region, situation may go out of hand in due course. For example, inflows into the Saguling reservoir in Indonesia have been decreasing over a period of time. Due to watershed degradation the dry season flow into the reservoir was found to be around 38 percent of the average annual flow between 1986 and 1991. This percentage went further down to 36 percent between 1992 and 1997 and to 34 percent in 1998 (ADB, 2006). Therefore SEA helps in preparing an early warning system for taking care of the environmental concerns. With an effective environmental policy and legislative framework in place, Thailand has been paying due attention towards its environmental requirements and focusing on environmental and energy concerns at the national and provincial levels. But, the environment management faces a stiff challenge in view of the rapid industrialization in the country. The country is mainly divided into 7 river basins with further sub-division into 25 drainage areas1. In order to manage the watershed region, the river basins and drainage areas have to be kept in good shape so that the charging system of earth is not blocked. Thailand has forestland stretching over 2000 kilometers from north to south with varied climatic conditions and a coastline. The clear and muddy waters too lap productive mudflats (Datamonitor, 2009). The Strategic Environmental Assessment (SEA) is therefore meant to serve as a promotional tool for expanding the adoption of policies towards preserving the watershed areas. Thailand shares three rivers with its neighbors Myanmar, Malaysia and the Lao People's Democratic Republic. The strategic environment assessment calls not only for assessing the status of watershed areas, but it also requires the experts to consider the management of all the relevant stakeholders. Some of the key stakeholders in this venture include landowners, local businesses, Farmers, industries, city officials, environmental activists etc. The diversity of these groups and their interests often creates a situation where it becomes very difficult to carry out the maintenance of the land, vegetation and water resources of the drainage basin. If the strategic policies are

Analysis of Mental Shortcuts Essay Example for Free

Analysis of Mental Shortcuts Essay Being creatures gifted with reason, people are always thinking and trying to rationalize things. For each person and situation, there are different kinds of ways of thinking or mental shortcuts. One of them is Representativeness heuristic. This refers to the way a person thinks according to his insticnts and past experiences. Unlike other modes of thinking, representativeness heuristic consumes less time. It does not involve much cognitive activity. In this mode of thinking, people tend to refer to the past for decision making and judgment of occurences or events (Akent et al, 2007). Another type of type of heuristic is the anchoring and adjustment heuristic. This type of heuristic greatly relies on an anchor or initial opinion or thought about a certain object, place, or situation. Despite having other evidence before the judgment, the judgment and the trail of thought of the person remains to be hanging on to the initial thought. Unlike representativeness heuristic, this mode of thinking is slower in that more opinions are gathere before a decision is made and the rationalization concluded (Akent et al, 2007). The third kind of heuristic is the availability heuristic. This type of heuristic make use of examples for making a decision or judging an event or occurence. In addition, availability heuristic the frequency of an event may be predicted through the said example. As compared to the first two kinds of heuristic, this may be slower as there is a need to gather enough examples as basis for decision to be made. If there are no examples at hand, another mode of thinking may be utilized(Akent et al, 2007). If carefully analyzed, these modes of thinking are all faster as compared to the normal flow of thought in a persons mind. However, if measured and checked for results, all can be accurate and fast given a specific situation. For instance, the availability heuristic is faster if there are examples at hand. However, without them the other types of heuristic will prove to be more accurate and faster. Thus, all three mental shortcust prove to be helpful in a particular situation in life but not in its entirety. References Elliot Aronson, Timothy D. Wilson, and Robin M. Akert. (2007). Social psychology (6th ed. ). Prentice Hall

Nuclei And Mitochondrial Fraction

Nuclei And Mitochondrial Fraction The objective of this experiment is to prepare a nuclei and mitochondrial fraction using differential centrifugation, from a rat liver homogenate sample. The amount of activity of mitochondria in the fractions can be measured using succinate dehydrogenase (SDH) as a marker. To measure the percentage recovery of the SDH of Mitochondrial, Nuclei and supernatant fractions in comparison to the Homogenate and to Calculate the specific and relative activity of SDH in each fraction. Figure 1: Shows a typical animal cell with the individual organelle components. Figure 2: Shows the typical features and functions of the organelles of interest in this report. Figure 1 + 2 Created on Microsoft paint with reference to Essential Biology (2004) Individual organelles differ in size but are all usually around 10nm in diameter. There is a small surface area and size/density depends on the organelle, the smaller organelles being lysosomes and ribosomes. Mitochondria differs in cell type depending on the energy demand of that organ, the more ATP that is required in a particular organ the more mitochondria found. E.g. more mitochondria found in heart and liver cells than in a white blood cell like a lymphocyte. Smaller organelles include lysosomes and ribosomes. Metabolism can be detected using various methods such as use of inhibitors. These can be both competitive and non-competitive, an example is seen with arsenic with inhibits pyruvate dehydrogenase. Another method is with the use of radioisotopes to measure activity aswell as histochemistry, immunocytochemistry and electromicroscopy. Preparation of the homogenate occurs in various stages. Firstly the homogenisation of liver cells. This can be done using a Potter Elvehjem homogeniser to extract organelles without damaging the actual cell. This is a simple and effective homogenisation method. A small gap is made within the cell wall which is then pressurised which forces the contents i.e organelles, cytoplasm etc. out of the cell. This occurs at a low temperature and mild pH, and to keep the isotonic solution a sucrose buffer is used, therefore since there is the same water potential inside the cell and outside the cell there is no net movement of water (osmosis) and thus the cell remains the same size. Homogenized cells also must be kept at low temperatures to prevent autolysis (the degradation of a cell by its enzymes). (www.bookrags.com). Figure 3 shows a classic Potter Elvehjem homogeniser Image taken from (umwcellbiology.org) The second stage is fractionation of the homogenate sample. This process is called centrifugation and can be further split into either a differential centrifugation or a density gradient centrifugation. The differential centrifugation splits the impure fraction into separate compartments due to the size of the various organelles in question and there density. The centrifuge applies a gravitational force onto the sample to separate components. The rate of centrifugation is determined by the acceleration or speed applied to the homogenate and is usually measured in revolutions per minute (RPM) or g. Depending on the density of the organelles will determine their isolation at a given speed. The higher density organelles and the bigger organelles separate at a lower speed centrifugation. (K. Wilson 2005). The separation forms a pellet which is the precipitate proportion of the sample and the component of interest and a supernatant which is the liquid component. The supernatant readily de canted from the sample without removing the precipitate. Diferemces in centrifugation occur due to the techniques used, differential centrifugation is based upon the sedimentation rate of particles and thus the sedimentation rate separates them based on size and density. After initial sedimentation the largest particles separate first into pellet and supernatant (K. Wilson 2005). Density gradient centrifugation separates organelles using a media. Various media can be applied and depending on the particles will be best for certain types and may not work well for others. (K. Wilson 2005). The 4 fractions we will obtain are nuclei, mitochondrial, supernatant and homogenate. Various tests can be carried out to distinguish between fractions and to determine their actual purity, testing for specific enzymes can code for the activity occurring in the cell fractions therefore indicating the most abundant component. Some tests include: Testing for DNA in both nuclei and mitochondrial fractions. This is because DNA is contained within the nucleus but also within the mitochondria. This is because relating to the endosymbiotic theory mitochondria was a separate aerobically respiring bacterial cell which was later engulfed by an early eukaryotic cell to merge into one aerobically respiring cell. Mitochondria is maternally inherited in the case of the majority of multicellular organisms, this is due to the higher number of mtDNA molecules in the ooecyte and much fewer in a sperm cell which are mostly degraded before fertilization takes place. Test for histones which indicate nuclei fraction as well as testing for various enzymes such as ATPase found in cytoplasmic (supernatant) and mitochondrial fractions and phosphotase kinase indicating microsomes and golgi apparatus are present. Some enzymes are exclusive to the citric acid cycle which occurs in the mitochondria, therefore testing for these enzymes indicates the presence of mitochondria in a fraction. The enzyme marker to test for mitochondria which we use is succinate dehydrogenase which is exclusive to the inner mitochondrial membrane. Succinate dehydrogenase is formed only during the citric acid cycle so is only given as an indication of mitochondria. However, since during the homogenisation process the mitochondria could potentially burst spilling their contents into the cytoplasm (supernatant fraction), this does not therefore give an accurate indication of mitochondria present in a fraction. Succinate dehydrogenase breaks down succinate into fumurate, therefore t he measurement of formazan indicates presence of succinate dehydrogenase. Measuring Succinate Dehydrogenase Activity (Red Formazan assay) This occurs in 2 reactions: 1: succinate + FAD à ¨ fumarate + FADH2 SDH breaks down succinate into fumarate. This is an oxidation reaction since the succinate loses 2 electrons, in addition a reduction of the enzyme flavin adenine dinucleotide occurs (FAD gains 2 electrons) (FAD + 2 electrons à ¨ FADH2) Figure 4: Shows the redox reaction which occurs with succinate and FAD. Image taken from natuurlijkerwijs.com SDH activity is measured by the formation of formazan a deep red compound formed from the reduction on a tetrazolium salt. The reduced FADH2 reduces tetrazolium salt (INT). 2: FADH2 + INT à ¨ FAD + formazan Centrifugation and calculating the relative centrifugal field. (K. Wilson 2005) G = W2r = 4 II2 r (rPM)2 = 1.1110-5r (rPM)2 3600 G= Relative centrifugal force (RFC) r = Radical distance from axis of rotation w = Angular velocity rPM = Revolutions per minute. T = 9 É ² (In Rt/Rb) 2 W2rp2 (Pp -P) É ² = Viscosity of medium rp = Radius of particle Pp = Density of particle P = Density of medium Rt = Radius to top of centrifuge tube Rb = Radius to bottom of centrifuge tube. There are many differences in types of centrifuges available and results depend on the speed of the centrifugation and whether a vacuum is present and the type of rotor used. (K. Wilson 2005) Analysis of marker enzymes in subfractions determines the recovery of subcellular organelles, with comparison to previous tests, quantative data can be used to assess contamination of fractions. Showing whether the subfractionation method has been successful or not. These tests also hold health benefits and implications e.g. microsome C causes cell death and can be found in mitochondrial fractions, however in cancer patients no microsome c is present, indicating no cell death will occur a common feature of cancer cells. Enzyme measurement in subcellular fractions however does hold some implications such as the solubility of the environment which may cause differences in enzyme function. Another implication is latency of enzymes, this refers to whether proteins are bound to the enzyme which in turn activates them once bound signalling enzyme function. There may also be low recovery of enzymes in the fractions due to poor recovery of the organelles which they come from, in particular if the enzyme is confined only to a specific region. Over the 3 week period centrifugation will separate the fractions according to size/density and separating the sample into the pellet and supernatant fractions. The speed of the centrifuge determines whether the pellets will separate. A lower speed is needed to separate the nuclei fraction due to the higher density, whereas the higher speed is needed to separate the supernatant due to the smaller density remaining organelles. (K. Wilson 2005). The protein content is also measure for each fraction using the biuret assay, absorbance values are given which determine the protein content of each fraction. Finally succinate dehydrogenase is measured. This causes a redox reaction and causes e- ions are released, using formazan as an indicator this changes the colour of solution red, showing a redox reaction has taken place. From this research I can predict that the mitochondrial fraction is expected to have the highest results in specific activity due to fewer proteins present in that fraction. Results: Calculations: Formazans molar extinction coefficient (E490nm) = 20,100 M-1 cm-1 The specific activity and relative activity of the fractions can be determined by measuring the concentration using Beer- Lamberts Law: (www.chemguide.co.uk) A = ÃŽ µ x l x C A = Absorbance (no units) ÃŽ µ = Epsilon. The adsorbtion coefficient M -1 cm -1 l= Cuvettes light path length, this is the length of solution a light passes through (always 1 cm) C= Concentration of substance in M (moles in 1 litre) Rearrange to give concentration: C = A / ÃŽ µ x l Units: M-1 x cm-1 = 1 / M x cm C = A / ÃŽ µ x l Gives units: ( 1/ (1/M x cm) x cm). This can be simplified to give 1/ (1/M) And further simplified to give units: M (moles per litre or dcm -3) Know values: ÃŽ µ = the formazan adsorption coefficient is 20,100 M -1 cm -1 A = refers to the absorbance at 490nm values for each fraction are found in the mean-control table section. Using the equation: C = A / ÃŽ µ x l We can work out the concentration of formazan formed in the reaction. The concentration value is for 1 litre, therefore we must calculate the actual concentration from the actual assay volume used. Concentration = amount/volume rearranged to give A = C x V The final assay volume from week 3 is 6 ml* due to the addition of ethyl acetate. * Note by mistake 6ml of ethyl acetate was added instead of 4 ml giving a different final volume to the other groups. Converting 6ml into its litre value and x by the concentration gives the accurate mole product of formazan produced. Reaction time needs to be included to give the accurate units. Activity units can be determined using the following equation. Activity = Moles of formazan/reaction time (12 minutes) This gives the activity in M -1 Calculating total activity and specific activity of the fractions. Table 1: Total volumes from each cellular fraction. Fraction Total Volume (ml) Homogenate 12 Nuclei Fraction 12 Mitochondrial Fraction 12 Supernatant Fraction 26 To do this we need to take into the account: The total volume The total protein of the fraction. Dilution factor The total volume values for each fraction can be found in table 1. The sample of each fraction used was 0.2ml, therefore the amount of moles of formazan calculated is in 0.2ml. (0.2 / total volume) x moles of formazan in 0.2ml X by the dilution factor of each fraction to give the total activity for each fraction, the values are given in table 4. To determine the specific activity we must consider the total protein of the fraction. Values are given in table 3. Specific activity = Total activity of fraction/ total protein of fraction Table 2: Bovine serum albumin (BSA) solution concentrations Volume (ml) BSA (10mg/ml BSA 0 0.2 0.4 0.6 0.8 1.2 1.6 0.1m NaOH 2.0 (blank) 1.8. 1.6 1.4 1.2 0.8 0.4 Table 3: Values for BSA standard curve. See Graph 1 for the results from the corresponding fraction absorbance. Protein Amount (mg) 0 (blank) 2 4 6 8 12 16 Absorbance at 550nm 0 0.105 0.184 0.275 0.354 0.511 0.531 Table 4: Protein amount in homogenate and subcellular fractions. Homogenate 0.05ml Nuclei 0.2ml Mitochondria 0.2ml Supernatant 0.2ml Average Absorbance (550nm) 0.169 0.054 0.174 0.199 Protein amount in samples aliquot (mg) 3.6 1.18 3.8 4.15 Protein concentration in fraction (mg/ml) 72 5.9 19 20.75 Protein amount in fractions total volume (mg) 864 70.8 228 539.5 Graph 2: Shows the difference in protein amount amongst cellular fractions. Table 5: Actual concentration of fraction after dilution. Dilution Factor Actual concentration (mg/ml) Homogenate 20 3.6 Nuclei 3 2 Mitochondrial 20 0.95 Supernatant 1 20.75 Table 6: Formazan content absorbance at 490nm. Fraction Control Test 1 Test 2 Mean-Control Homogenate 0.132 0.58 0.52 0.42 Nuclei 0.21 0.352 0.326 0.13 Mitochondrial 0.057 0.391 0.265 0.27 Supernatant 0.132 0.52 0.33 0.29 Results for Homogenate: From table 5, we have the absorbance of homogenate as 0.42 this divided by the adsorption co-efficient gives: 0.42/20,100 = 2.1 x 10 -5 M The units for concentration are left as moles per litre (M). To get this into moles in the actual volume used (6ml not 1 litre) 2.1 x 10 -5 M x 0.006 lite = 1.3 x 10-7 M Include the reaction time of 12 minutes to give moles per minute. 1.3 x 10-7 M /12mins = 1.010-8 M -1 To determine total activity and specific activity. The total volume from table 1: for the homogenate is 12ml, however the sample used was only 0.2ml we therefore divide actual volume / used volume x concentration of H x dilution factor (20 in the case of the homogenate from table 5 values) Total activity = (12/0.2) x1.0x10-8 M -1 x 20 = 1.2 x 10 -5 M -1 specific activity = 1.2 x 10 -5 M -1/ total amount protein in homogenate from table 4 1.2 x 10 -5 M -1/864= 1.3 x 10-8 M min-1 Results for nuclei fraction: 0.13/20,100 M-1 cm-1 = 6.5 x 10-6 In 0.006 litre : 6.5 x 10-6 x 0.006 = 3.9 x 10-8 M 3.9 x 10-8 M / 12 = 3.2 x 10-9 M min-1 Total activity = 3.2 x 10-9 M min-1 x (12/0.2) x 3 = 5.8 x 10 7M min-1 Specific activity = 5.8 x 10 7/ 70.8 = 8.2 x 10 -9 M min-1 Results for mitochondria: C = 0.27/20,100 m-1 cm -1 = 1.3 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM 1.3 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM x 0.006 = 7.8 x 10-8 M 7.8 x 10-8 M / 12 = 6.5 x 10-9 M min-1 Total activity = 6.5 x 10-9 M min-1 x (12/0.2) x 20 = 7.8 x 10-6 M min-1 Specific activity = 7.8 x 10-6 M min-1/228 = 3.4 x 10-8 M min -1 Results for supernatant: C = 0.29/20,100 m-1 cm -1 = 1.4 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM 1.4 x 10-5à ¢Ã¢â€š ¬Ã¢â‚¬ËœM x 0.006 = 8.7 x 10-8 M = 8.7 x 10-8 M / 12 = = 7.3 x 10-9 M min-1 Total activity = 7.3 x 10-9 M min-1 x (26/0.2) = 9.4 x 10-7 M min-1 Specific activity = 9.4 x 10-7 M min-1/539.5 = 1.7 x 10-9 M min -1 Percentage recovery of Succinate Dehydrogenase for the fractions This is done by dividing the amount of Succinate dehydrogenase in the individual fractions by the original homogenate and then multiplied by 100 to give a percentage. Table 7: Shows the total activity for each of the fractions. Fraction Total Activity Homogenate 1.2 x 10 -5 M -1 Nuclei 5.8 x 10 7M min-1 Mitochondria 7.8 x 10-6 M min-1 Supernatant 9.4 x 10-7 M min-1 Nuclei fraction: (5.8X10-7/1.210-5 ) x 100 = 4.8% Mitochondria fraction (7.810-6/1.210-5 ) x 100 = 65% Supernatant fraction (9.410-7/1.210-5) x 100 =7.8% Relative Specific Activity of Succinate Dehydrogenase This is found by dividing the specific activity of the fractions (found above) by the specific activity of the homogenate (found above). Table 8 shows the specific activity for each of the fractions: Fraction Specific Activity Homogenate 1.4 x 10-8 M min-1 Nuclei 8.2 x 10 -9 M min-1 Mitochondria 3.4 x 10-8 M min -1 Supernatant 1.7 x 10-9 M min -1 Nuclei fraction 8.2 x 10 -9 M min-1 /1.4 x 10-8 M min-1 = 0.586 Mitochondrial fraction 3.4 x 10 -8 M min-1 /1.4 x 10-8 M min-1 = 2.429 Supernatant fraction 1.7 x 10 -9 M min-1 /1.4 x 10-8 M min-1 = 0.121 Discussion: Note: There was very little protein found in the nuclei fractions total volume, this is abnormally low since we would expect this to be higher. From the results we can determine that the this supports our prediction that The mitochondrial fraction is expected to have the highest results in specific activity due to fewer proteins present in that fraction. Organelles have been isolated from each other as seen with the differing proportions of protein found in each fraction as well as the differing values for specific and total activity calculated. However the homogenate is expected to have the highest total activity due to the higher amount of protein since all fractions are present. However since protein was found in the cytoplasm or supernatant fraction, this indicates that there was an error in the separation of the fractions as SDH is present where it usually isnt found. Succinate dehydrogenase works by transferring 2 electrons from succinate which transfers it to fumerate, which blocks the rest of the reaction when it binds to FAD, from the measurement of formazan gives the value of activity. Results show that the relative specific activity is highest in the mitochondrial fraction, as well as the percentage recovery of the fractions. Therefore demonstrating that the fractions were purified and that the homogenisation and centrifugation has been relatively successful in separating fractions. However there were some inaccuracies from the results, this includes the very low protein amount found with in nuclei fraction, this was however predicted to contain a higher amount of protein due to the nature of the organelle and the enzymes contained within it. Another inaccuracy in this experiment is that SDH was found within the supernatant. This is primarily a marker for mitochondria so would not usually be found within the cytoplasm, however due to mitochondria bursting and releasing its contents into the cytoplasm during the homogenisation stage and centrifugation the enzyme succinate dehydrogenase was present. Since the test was carried out under the same conditions in a neutral pH buffer we can conclude that this was a fair test, however is it often found that the more molecules present in a The separating of the homogenate could be improved by using another method of homogenisation, in this experiment we used a Potter Lethem homogeniser which is a glass and plastic hand homogeniser. This perhaps isnt the most accurate at pressurising cells with the force needed to accurately release cell content. Alternative homogenisers include ultrasonic and rotor based homogenisers which may provide more accurate. (www.proscientific.com) A different centrifugation method used. During this experiment differential centrifugation was used, however density gradients may provide more accurate at purifying a sample (www.coleparmer.co.uk). This method works by placing various layers after layer of gradient media such as sucrose in a tube with the heaviest layer at the bottom and the lightest at the top. The cell fraction to be separated is placed on top of the layer and centrifuged. Density gradient separation can be classified into two categories. Rate-tonal (size) separation. Isopycnic (density) in which organelles separate until their density matches the surroundings of the media in which they are. A very good medium for separating organelles is an iodinised media. (www.coleparmer.co.uk). Accuracy of the absorbance and accuracy of obtaining the protein amount. Results are slightly low indicating inaccuracy in both collecting the samples and also measuring the absorbance, this could be due to error in homogenisation and centrifugation techniques but could also be due to error in the reading of absorbance using the Spectrophotometric assay since U.V wavelength has different absorbance levels if either oxidised or reduced enzymes absorb light therefore giving innacurate indication to enzyme present (www.millipore.com) . This may affect the absorbance levels in the fractions if specific enzymes are affected thus giving an altered absorbance level and therefore undermined protein amount. Another method to measure enzyme assay could be to use a caliometric method which measures heat radiance given off instead of the absorbance levels. Some of the organelles which remain in the supernatant fraction are the smaller and less dense proportions of the cell such as ribosomes and lysosomes. Further centrifugation at a higher speed can be used to separate these smaller less dense organelles into pellets. This can also be used to further purify bacteria. In conclusion we see that as predicted, the specific activity is highest in the mitochondrial fraction and the total activity is highest in the homogenate. The % recovery of each fraction and the relative specific activity for each fraction calculated shows a higher proportion in the mitochondrial fraction also. Overall the results indicate accurate laboratory skills and results conclude what was intended, however some slight changes to laboratory equipment would mean that some of the results such as SDH found in the supernatant may not come about in a future test.